Potential benefits of Rehmanniae Radix after ancient rice‐steaming process in promotion of antioxidant activity in rats' health

Rice steam processed product of Rehmanniae Radix (RSRR), one the products (RR), is popular as an herbal medicine and food. However, health-promoting effects mechanisms RSRR are still unclear. In this study, 10-week-old Sprague–Dawley female rats were treated with different RR. No organ coefficient differences observed between control group, indicating that did not cause damage to rats. Compared other RR products, superoxide dismutase, glutathione, catalase levels significantly higher malondialdehyde lower in exerted a better antioxidant effect. Gene expression analysis showed hemoglobin genes (Hba-a1, Hba-a2, Hbb-bs, Hbb, Hbq1b, Hbb-b1, LOC103694857) may be potential biomarkers evaluate effect RSRR. Antioxidation-related signaling pathways GO annotation, including cellular oxidant detoxification, hydrogen peroxide metabolic process, complex, oxygen binding enriched, these represent mechanism To explore main active compounds primarily responsible for activity RSRR, UPLC-Q-TOF-MS was used six components (catalpol, rehmannioside A, D, melittoside, ajugol, verbascoside) identified rat serum. Catalpol A predicted major by network pharmacology. These results suggested exhibits has properties. This study provides scientific basis clinical use